Rapid Detection of COVID-19 by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method
Authors
Abstract:
Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was examined by LAMP-RT PCR and Real-Time RT- PCR techniques. The nasopharynx and oropharynx nucleic acids were extracted both by pishtaz RNA extraction kit. LAMP-RT-PCR was studied with direct samples, but Real-Time RT-PCR samples were tested only by RNA extraction samples. The probe-primer mixture of this kit is designed by a dual-target gene method that simultaneously targets the genomic sequences of the spike region and N nucleocapsid. Fluorescence was measured by Real-Time RT-PCR and LAMP-RT PCR. Results: Many 200 samples of nasopharynx and oropharynx collected from Jamaran Heart Hospital were studied. Clinical specimens (56%) were positive by Real-Time RT-PCR technique with mean Ct values less than 30. In the RT-LAMP technique of RNA extraction samples, clinical samples (52%)were positive for Covid-19. RT-LAMP technique indicated a sensitivity of 92.8%. Also in the RT-LAMP method without RNA extraction, the sensitivity of the technique was 85.7%. Conclusion: The LAMP-RT PCR technique is emerging as an appropriate alternative to the Real-Time RT-PCR method. This technique has some basic advantages such as constant temperature amplification, thermal cycle elimination, faster results, and potentially greater detection capacity. Therefore, the RT-LAMP-PCR can be used as a quick and cheap technique that can be used in all areas.
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Journal title
volume 17 issue 3
pages 3- 3
publication date 2023-06
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